Figure 1
From: The role of the MAPK pathway alterations in GM-CSF modulated human neutrophil apoptosis with aging
Measurement of human neutrophils apoptosis with aging by flow cytometry. A) GM-CSF-treated (20 ng/ml) or untreated neutrophils were cultured during various time periods (from t = 0 to t = 18 h) and stained with Annexin-V conjugated to FITC. FACS analyses were performed for fluorescence intensity and data were reported in a time-dependent manner for the different experimental conditions and compared to control neutrophils from young (white columns) and elderly donors (black columns). Identical experiments were done with Z-DEVD-FMK, inhibitor of caspase-3 activity (iC3) in the presence or absence of GM-CSF. Data represent percentage of apoptotic cells from the neutrophil pool. Data were statistically analyzed for 20 different donors of each group indicated by *p < 0.01. B) The same experiments were done using H2O2 (250 μM), Dexamethasone (10-8 M) and FMLP (10-8 M). Data are from 10 independent experiments with *p < 0.01.