Figure 5
From: The role of the MAPK pathway alterations in GM-CSF modulated human neutrophil apoptosis with aging
Expression of bcl-2 family members Bax and Bcl-xL in PMN with aging upon treatment with GM-CSF and PD98059 and SB203580 for 18 h. Neutrophils were cultured in presence of GM-CSF alone or in combination with either the p42/p44 MAPK inhibitor, PD98059, or the p38 MAPK inhibitor, SB203580, for 18 h. Then, neutrophils were lysed and western-blotting experiments were executed to investigate the amount of Bax in young (A) and elderly donors (B). The same experiments were conducted for studying Bcl-xl expression in PMN of young (C) and elderly subjects (D). GM-CSF significantly decreased the expression of Bax in PMN of young subjects (A, p < 0.01), without any effect in PMN of elderly subjects (B). GM-CSF significantly increased the expression of Bcl-xL (p < 0.05) in both age groups (C and D), Blots are representative of 15 independent experiments.