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Table 1 Neutrophil apoptosis modulation by MAPKinase inhibitors

From: The role of the MAPK pathway alterations in GM-CSF modulated human neutrophil apoptosis with aging

Treatment

Culture time (hours)

Annexin-V positive cells (%)

  

Young

Elderly

 

0

11 ± 2

15 ± 3

None

6

11 ± 3

20 ± 3

 

18

34 ± 6

48 ± 10

GM-CSF

6

11 ± 4

10 ± 5

 

18

18 ± 3

41 ± 9*

PD98059

18

33 ± 7

45 ± 10

GM-CSF + PD98059

18

40 ± 10

50 ± 15

SB203580

18

43 ± 2

53 ± 5*

GM-CSF + SB203580

18

24 ± 3

33 ± 5*

  1. The cells were cultured for the indicated time in the presence or absence of GM-CSF (20 ng/ml) and the presence or absence of PD98059 (30 μM) or SB203580 (10 μM). The percentage of apoptotic was determined by labeling with FITC-conjugated Annexin-V and cell integrity, by Propidium iodide (PI) staining. The cells were analyzed by cytofluorimetry. Data are shown as the mean ± SD of five 5 independent experiments. Significant differences between young and elderly subjects are indicated by an asterisk (*, p < 0.05 and **, p <0.01).
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Immunity & Ageing

ISSN: 1742-4933

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