Figure 1
From: The effect of aging and caloric restriction on murine CD8+ T cell chemokine receptor gene expression
Murine CD8+ T cell chemokine receptor gene expression. Autoradiographs of representative ribonuclease protection assay (RPA) comparing young (Y) (3–4 months old) and old (O) (18–20 months old) C57BL/6 CD8 T cell CCR1–5 (A) and CXCR2, 4 and 5 (B) gene expression. Composite histogram (C) of 5 RPAs using pooled RNA from a total of 25 mice in each age group is shown. The quantitation was done by measuring the P32-radiation from identical size band areas on the RPA gel using a Phosphoimager. The results represent the mean ± SEM of the chemokine receptor gene expression level of old CD8+ T cells relative to young CD8+ T cells (arbitrarily defined as equal to 1). Gel loading is corrected with L32 expression. P value of ≤ 0.05 is considered statistically significant. * P ≤ 0.05, ** P ≤ 0.005, *** P ≤ 0.001, NS = not significant.