SENIEUR status of the originating cell donor negates certain ‘anti-immunosenescence’ effects of ebselen and N-acetyl cysteine in human T cell clone cultures

Table 2 GSH:GSSG ratio of TCCs + / - NAC

Time in culture (Weeks)	7.5 mM NAC	400-23 (26 yr old)	385-7 (45 yr old)	399-37 (80 yr old)
2	-	46.3 ± 2.5	44.2 ± 0.8	36.7 ± 1.0*
2	+	51.2 ± 4.5	46.7 ± 3.3	35.7 ± 0.9*
5	-	37.9 ± 0.8	41.0 ± 0.5	29.4 ± 1.2*
5	+	42.5 ± 2.5	46.1 ± 0.3	30.7 ± 1.8*
9/10	-	35.5 ± 4.0	33.2 ± 3.1	24.2 ± 0.2*
9/10	+	35.8 ± 2.3	36.6 ± 2.6	24.1 ± 0.2*

GSH:GSSG ratios in three pooled CD4⁺ TCCs (+ / - 7.5 mM NAC) derived from a healthy 80 year old donor are significantly lower than the levels in either of the three pooled TCCs, each derived from a healthy 26 or a 45 year old donor (published data from [10]).
*Significantly lower GSH:GSSG ratio in 399-37 clones (80 year old) compared to either 400-23 (26 year old) or 385-7 (45 year old) clones.

ISSN: 1742-4933