Fig. 2

Dsg3H1-Rag2^−/− T cells were more activated and showed higher Birc5 expression in aged mice. a, b FCM plots and quantitative summaries of the expression of the indicated factors in Dsg3H1-Rag2^−/− T cells in SLNs on day 3 after transfer into aged and young mice (gated on Ly5.1⁺CD4⁺ cells; The gating strategy is shown in Fig. S2a). c Quantitative summaries of the OX40L expression levels in four subsets of migratory DCs in SLN of aged and young mice: Langerin⁻CD11b⁻, Langerin⁻CD11b⁺, Langerin⁺CD103⁻, and Langerin⁺CD103⁺ cells. The gating strategy is shown in Fig. S2b. d, e FCM plots and quantitative summaries of OX40 expression in CD4⁺Foxp3⁻ non-Tregs and CD4⁺Foxp3⁺ Tregs in SLNs in aged and young mice without transfer of Dsg3H1-Rag2^−/− T cells (The gating strategy is shown in Fig. S2c). Data are shown as the mean ± SEM. ns, not significant, *P < 0.05, **P < 0.01 (unpaired t-test between groups). Data were pooled from three independent experiments (n = 3–5 mice per group) for a, b. Data are from two independent experiments (n = 3–5 mice per group) for c-e