Fig. 6

DN T cells express tissue residency markers and intracellular cytotoxic molecules. A Representative contour plot of CD69 and CD103 expression on DN T cells. B Percentage of CD69 + DN T cells in different anatomical regions of the FRT (EM = 28, CX = 19, ECX = 22). C Lack of correlation between CD69 + DN T cells and age in endometrium (EM = 28), endocervix (CX = 19) and ectocervix (ECX = 22). D Representative overlay histogram of CD103 expression by DN T cells (black), CD8 + (gray) and CD4 + (clear) T cells. E Percentage of CD103 + DN T cells in different anatomical regions of FRT (EM = 24, CX = 15, ECX = 14). F Lack of correlation between CD103 + DN T cells and age in EM (left), CX (center) and ECX (right). G Representative plots of Perforin (right), Granzyme B (center) and Granzyme A (left) expression by DN (black), CD8 + (gray) and CD4 + (clear) T cells in the FRT. H Comparison of percentage of CD103 + (black) and CD103- (white) DN T cells expressing perforin (PF), granzyme B (GZB) and granzyme A (GZA) in the endometrium (EM = 12), endocervix (CX = 10) and ectocervix (ECX = 10). Wilcoxon paired test was used to compare CD103 + and CD103- DN T cells within each tissue. *p < 0.05, **p < 0.01, ***p < 0.001. I Correlation between age in postmenopausal women and perforin + CD103 + DN T cells and (J) perforin + CD103- DN T cells in EM (N = 8), CX (N = 9) and ECX (N = 9). Spearman correlation analysis was performed. Each dot represents a different patient