Fig. 2

The infiltrations of BMDMs in brain after parabiotic surgery. a Immunofluorescence image of microglia and BMDMs co-stained with CD68, IBAI, and P2Y₁₂ antibodies in neocortex and hippocampus of Wt mice brain at the age of 11 months. Scale bars: 500 µm. b Immunofluorescence image of microglia and BMDMs co-stained with CD68, IBAI, and P2Y₁₂ antibodies in neocortex and hippocampus of Tg mice brain at the age of 11 months. Scale bars: 500 µm. c The statistical diagram of the amounts of BMDMs in neocortex and hippocampus of Wt and Tg mice brain at the age of 11 months. The amounts of BMDMs in neocortex and hippocampus of Tg mice were significant higher compared with Wt mice at the age of 11 months (p = 0.000). d Immunofluorescence image of microglia and BMDMs co-stained with CD68, IBAI, and P2Y₁₂ antibodies in neocortex and hippocampus of PaWt(Wt-Wt) mice brain at the age of 11 months. Scale bars: 500 µm. e Immunofluorescence image of microglia and BMDMs co-stained with CD68, IBAI, and P2Y₁₂ antibodies in neocortex and hippocampus of PaWt(Wt-Tg) mice brain at the age of 11 months. Scale bars: 500 µm. f The statistical diagram of the amounts of BMDMs in neocortex and hippocampus of PaWt(Wt-Wt) and PaWt(Wt-Tg) mice brain at the age of 11 months. The amounts of BMDMs in neocortex and hippocampus of PaWt(Wt-Tg) mice brain were significantly higher than PaWt(Wt-Wt) mice at the age of 11 months (p = 0.004). 5 images were taken per mouse and Image J used to count the amounts of BMDMs. (n = 6, Scale bars 500 µm. ***P < 0.001, **P < 0.01, ns denotes no statistical significance)