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Fig. 3 | Immunity & Ageing

Fig. 3

From: Systemic immune response in young and elderly patients after traumatic brain injury

Fig. 3

Analysis of lymphoid and myeloid cell populations. Percentage of yield (a) and viability (b) after thawing of PBMC from young (circles) and elderly (triangles) donors, control (white symbols) and TBI patients (black symbols). (c) Percentage of different immune subsets evaluated by flow cytometry. T cells were identified as CD3+, B cells as CD19+, NK cells as CD56+, and monocytes as CD14+ cells, in the morphological gate, after exclusion of dead cells and doublets. Pie chart of the mean of the percentage of different immune subsets was shown. (d) Results displayed in panel (c) are shown by separating TBI patients according to injury severity assessed as GCS score. * p < 0.05; ** p < 0.01; *** p < 0.001 TBI vs. control aged-matched subjects. §§ p < 0.01; §§§ p < 0.001 young vs. elderly control subjects

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