Fig. 6
From: Systemic immune response in young and elderly patients after traumatic brain injury
Analysis of CD4 Th subsets. (a) Gating strategy used to identify Th subsets: Th subsets were identified in the CD3 + CD4 + CD45RA- CD45R0 + gate, according to the expression of CD183/CXCR3, CD194/CCR4, CD196/CCR6, and CCR10 as follows: Th1 (CD194- CD196- CD183+ CCR10-), Th2 (CD194+ CD196- CD183- CCR10-), Th17 (CD194+ CD196+ CD183- CCR10-), Th1/Th17 (CD194+ CD196+ CD183+ CCR10-), Th22 (CD194+ CD196+ CD183- CCR10+), Th9 (CCR4- CCR6+), and ThGM-CSF (CCR4+ CCR6- CXCR3- CCR10+). Regulatory T cells (Treg) were identified as CD25hiFoxP3+. (b) Percentage of Th subsets is shown in PBMC from young (circles) and elderly (triangles) donors, control (white symbols) and TBI patients (black symbols). (c) Correlation between GCS score and the percentages of Th2 in young and elderly TBI patients. Correlations were assessed by Spearman correlation coefficients, and dashed lines represent 95% confidence intervals. (d) Percentages of Th2 subset are shown according to injury severity. * p < 0.05; ** p < 0.01 TBI vs. control aged-matched subjects. # p < 0.05 young vs. elderly TBI patients