Fig. 8
From: Systemic immune response in young and elderly patients after traumatic brain injury
Analysis of B lymphocyte subsets. (a) Gating strategy used to identify B cell subsets, in the gate CD19+, and based on the expression of CD27 and IgD, or CD24 and CD38, or CD24 and CD27, or CD27 and CD38. (b) Percentage of mature (IgD+ CD27-), non-switched memory (IgD+ CD27+), switched memory (IgD- CD27+), and double-negative B cells (IgD- CD27-). (c) Percentage of CD24- CD38- B cells. (d) Percentage of antibody secreting cells (ASC, CD27hi CD38hi)). (e) Percentage of regulatory B cells evaluated as CD24hi CD38hi (transitional B cells) and CD24hi CD27+. Analyses were performed in PBMC from young (circles) and elderly (triangles) donors, control (white symbols) and TBI patients (black symbols). * p < 0.05; ** p < 0.01 TBI vs. control aged-matched subjects