Table 2 Combinations and occurrence frequency of cell surface markers used to define B lymphocyte subpopulations
From: Association between body fat distribution and B-lymphocyte subsets in peripheral blood
Cell type | Surface markers | Input gate | % in ungated cells | % in CD19 + B cells | % in the input gate |
|---|---|---|---|---|---|
B cells | CD19+ | Lym | 3.28 (2.41; 4.44) | 100 (100; 100) | 10.96 (8.61; 13.88) |
Naive B cells | CD19 + CD27- IgD+ | CD19+ | 1.75 (1.20; 2.59) | 56.80 (45.58; 66.36) | 56.80 (45.58; 66.36) |
Marginal zone B cells | CD19 + CD27 + IgD+ | CD19+ | 0.45 (0.30; 0.68) | 14.03 (9.43; 20.19) | 14.03 (9.43; 20.19) |
IgM + B cells | CD19 + IgM+ | CD19+ | 1.73 (1.14; 2.50) | 53.87 (42.57; 64.63) | 53.87 (42.57; 64.63) |
IgD + B cells | CD19 + IgD+ | CD19+ | 2.32 (1.66; 3.24) | 73.44 (64.73; 79.36) | 73.44 (64.73; 79.36) |
IgM + IgD + B cells | CD19 + IgM + IgD+ | CD19+ | 1.49 (0.95; 2.19) | 45.99 (34.44; 57.89) | 45.99 (34.44; 57.89) |
IgM only B cells | CD19 + IgM + IgD- | CD19+ | 0.20 (0.12; 0.30) | 5.92 (4.00; 8.70) | 5.92 (4.00; 8.70) |
IgD only B cells | CD19 + IgM- IgD+ | CD19+ | 0.73 (0.46; 1.17) | 22.70 (15.11; 32.67) | 22.70 (15.11; 32.67) |
IgM- IgD- B cells | CD19 + IgM- IgD- | CD19+ | 0.66 (0.46; 0.96) | 20.48 (15.22; 26.79) | 20.48 (15.22; 26.79) |
Class-switched memory B cells | CD19 + IgM- IgD- CD27 + CD38low | IgM- IgD- | 0.39 (0.27; 0.58) | 11.88 (8.53; 16.78) | 60.12 (52.50; 67.51) |
Plasma blasts | CD19 + IgM- IgD- CD27 + CD38high | IgM- IgD- | 0.02 (0.01; 0.03) | 0.54 (0.31; 0.97) | 2.70 (1.60; 4.36) |
Class non-switched memory B cells | CD19 + IgM + CD27 + CD38- | IgM+ | 0.45 (0.31; 0.69) | 14.19 (9.68; 20.18) | 28.50 (18.22; 41.79) |
Transitional B cells | CD19 + IgM + CD27- CD24 + CD38+ | IgM + CD27- | 0.13 (0.07; 0.24) | 3.99 (2.28; 6.61) | 12.20 (8.20; 16.77) |
Memory B cells | CD19 + CD27 + CD38- | CD19+ | 0.97 (0.67; 1.40) | 30.38 (22.05; 40.64) | 30.38 (22.05; 40.64) |
IgD-CD27- B cells | CD19 + CD27- IgD- | CD19+ | 0.35 (0.21; 0.50) | 9.37 (6.84; 12.45) | 9.37 (6.84; 12.45) |
IgM + CD27- B cells | CD19 + IgM + CD27- | IgM+ | 1.23 (0.73; 2.00) | 35.96 (23.57; 47.90) | 69.26 (54.95; 79.60) |