Fig. 1

Summary of B-1 ATA B and B-2 AGcA cell generation, and mouse V_HV8-12 similar to human V2-5. A and B V_H8-12/V_k21 ATAμκ Tg mice generate B-1 ATA B cells in fetal/neonate stage and generate CD5⁺B cells as B1a, differently, negative selection (arrest) occurs during adult B-2 development. B-1 ATA B cells increase in PBL in middle age, and CD5 down can occur or continuous CD5⁺. In old age CLL/lymphoma generation, CD5^– cells mostly not increased ZAP70, and increased ZAP70 with CD5⁺ ATA B cells. TC^– (CD5^– and CD5⁺) increased CD11b (61/73=84%) in CLL/lymphoma, in contrast, Eu-TCL1⁺ Tg cells generated at middle aged CLL/lymphoma are few increased CD11b (13/60=22%) [13]. V_H8-12 ATAμTg mice can generate V_H8-12/V_k21 B1a and generate CLL/lymphoma in old age (low), and V_H8-12/V_k19 generate MZ B cells and V_H8-12/V_k19μκTg mice generated at 3 wk MZ B cells in B-2 and in old aged generate macrophage⁺⁺. C Mouse V8-12 VH check with human VHs from UniProt and IMGT. Mouse V8-12 V_H gene is similar to human V2-5