Fig. 3

Nod1 is important in early B1a cells and Nod1, IL-5R, CD1d decrease in old aged and increased CD11b and CXCR4, different from middle aged TC⁺Tg ATA B cells. A Nod1 high in neonate and adult B1a. 2 mo FO B versus pB1a for ZAP70 generation after 20 hour later by anti-IgM without or with CiE–DAP (Nod1) or MDF (Nod2), or TNFa without or with CiE-DAP. ZAP70^hi in a-IgM is only FO B cells not CD5⁺pB1a, but Nod1 add increased ZAP70. Down: 4 mo ATAμκ Tg mice with NOD1^+/– or NOD1^–/–. ATAid is originally generated V_H8-12/V_K21-5, then, low ATA B cells in Nod1^–/–. Thus, Nod1 is important. B Nod1, IL-5R, CD1d comparison between TC^– versus TC⁺Tg ATAμκTg, in 2 mo and meddle aged leukemia CLL and old aged TC^– CLL/lymphoma. Old aged TC^– tumor showed down these lists, not middle aged TC^– and TC⁺Tg. C Conclution of TC^–ATAμκTg ATA B in 2 mo verus old age tumor stage in spleen, with increased CD11b in TC^– ATA B in flow cytometry analysis. Down: In microarray analysis, TC^– old age ATA B cells are CXCR4 increase versus CXCR5 down, different compared with TC⁺Tg. Down right: Several TC^– versus TC⁺Tg ATAuk Tg lymphoma/leukemia pictures used for Fig. 4: 16 mo and 17 mo TC^–ZAP70^–CD5^– (Spl⁺⁺, mLM⁺⁺, LN⁺), 22 mo TC^–ZAP70⁺CD5⁺ (Spl⁺⁺, colon⁺⁺, LN⁺), 10 mo TC⁺ZAP70^–CD5⁺ (Spl⁺⁺, mLN⁺, PerC⁺⁺), 16 mo TC⁺ZAP70⁺CD5⁺ (Spl^++, Liver⁺⁺, PerC⁺⁺), and 12 mo samples Fig. 5