Fig. 1
From: Role of germinal center and CD39highCD73+ B cells in the age-related tonsillar involution
Age dependent distribution of tonsillar B-cell subpopulations. (A) Fresh TMC were stained for surface CD20, CD10, CD3, CD44, CD38, CD27 and a dead/live stain as in [8]. Samples were subsequently analyzed by FACS. The gating strategy to identify the different B-cell populations is partially illustrated. Singlets were gated by plotting FSC-H vs. FSC-A for each sample (not shown). Within the singlets population, dead cells were determined by a viability dye (not shown). Within the viable gate, lymphoid gate was determined through SSC-A vs. FSC-A (not shown). Dot plot depicting the representative percentage of the tonsillar B cells subsets for individuals from the youngest (top) and oldest (bottom) groups of patients analyzed. Percentages and colors designate frequencies of the populations indicated. (B) Histograms presenting the mean percentage ± SD of the BGC cell population frequencies determined as in A), from 76 individuals, distributed according their age. p value was calculated through unpaired t test, *p < 0.05; **p < 0.01