Fig. 3
From: Role of germinal center and CD39highCD73+ B cells in the age-related tonsillar involution
Age dependent distribution of tonsillar follicular T-cell subpopulations. (A) Fresh TMC were stained for surface CD4, CD8, PD 1, CXCR5 and a dead/live stain. Samples were subsequently analyzed by FACS. The gating strategy to identify the different follicular T-cell populations is partially illustrated and was performed as in [30]. Dot plots depicting the percentage of the tonsillar Tfh subsets of selected individuals, from the youngest (top) and oldest (bottom) groups of patients analyzed. Percentages and colors designate frequencies of the populations indicated. (B) Histograms presenting the mean percentage ± SD of the TfhGC and TfhM population frequencies determined as in A), from 55 individuals, distributed according their age. p value was calculated through unpaired t test, *p < 0.05; **p < 0.01