Figure 4

CpG-induced AID at t0 and the serum response are correlated. Frozen PBMC (10⁶ cells/ml), isolated from the peripheral blood at t0, were thawed and cultured with CpG, for 3 days. At the end of this time, cells were processed as described in Methods. Results are expressed as raw qPCR values (ΔCt) of AID mRNA normalized to GAPDH. Correlations between CpG-induced AID at t0 and H1N1-specific ELISA were calculated using GraphPad Prism 5 software. Pearson’s r = 0.4086 (left) and r = 0.5249 (right). P values are indicated for each graph. The numbers of individuals are given in Table 1.