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Table 1 Transition of memory T cells in BCG immunized mice with aging and retrieval effect of the boosting with Oligo-B on memory T cells $

From: Reactivation of immune responses against Mycobacterium tuberculosis by boosting with the CpG oligomer in aged mice primarily vaccinated with Mycobacterium bovis BCG

  CD4+ CD8+
Age Immunization CD44hi CD62L** CD44hi CD62L**
in mice in human equivarent* with BCG& low (TEM) high (TCM) low (TEM) high (TCM)
30-week late 10's + 72.2 ± 0.82 15.6 ± 0.80 61.7 ± 0.03 14.7 ± 0.49
- 74.5 ± 1.23 13.8 ± 0.35 48.2 ± 7.95 14.0 ± 3.67
81-90-week late 50's + 85.75 ± 5.91 2.85 ± 0.67 87.51 ± 6.94 2.07 ± 0.12
- 93.03 ± 0.56 5.95 ± 0.68 95.90 ± 0.82 3.38 ± 1.94
Boosting with Oligo-B      
30-week late 10's + 73.6 ± 1.92 17.3 ± 0.32 53.7 ± 10.3 9.95 ± 0.15
- 78.1 ± 3.89 14.6 ± 0.88 51.8 ± 0.48 10.2 ± 0.11
81-90-week late 50's + 89.12 ± 0.62 7.76 ± 3.26 95.29 ± 0.82 2.66 ± 1.08
- 85.82 ± 0.01 3.82 ± 0.42 92.07 ± 1.41 3.98 ± 0.12
  1. $Splenocytes were prepared from the aged mice indicated in the table and stained with specific antibodies to memory T cells (CD44, CD62L), and then analyzed by a flow cytometer. Precise procedures were described in the Section of Materials and methods.
  2. *The age in human equivalent was applied to survival curve of mice.
  3. &Mice were administered transdermally with BCG at 4-week old.
  4. **Effector memory T cell (TEM), CD44hi CD62Llo; central memory T cell (TCM), CD44hi CD62Lhi.
  5. N.D.: not determined.