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Table 3 Cytokines and chemokines in response to Aspergillus fumigatus allergen extract

From: Cytokine and chemokine responses to helminth and protozoan parasites and to fungus and mite allergens in neonates, children, adults, and the elderly

  IL-19 IL-27 IL-33 MCP-4 CCL-24 MIP-1 delta
Neonates 49 (0/603) 311 (0/1120) 62 (0/148) 0 (0/162) 283 (0/1198) 42 (0/87)
Children ND 1096 (957/1236) 97** (80/113) ND ND ND
Adults 280 (151/411) 823 (589/1056) 34 (7/60) 116 (72/162) 1728 (1474/1982) 29 (17/41)
Elderly 297 (172/424) 1136 (911/1363) 56 (32/81) 119 (75/163) 1558 (1315/1802) 31 (19/43)
  1. ** p<0.01 in comparison to adults for IL-33.
  2. Cellular production of interleukin (IL)-19, IL-27, IL-33 MCP-4/CCL13, MIP-1delta/CCL15 and Eotaxin-2/CCL24 in response to allergen extracts by umbilical cord blood cells from neonates (UCBC) or peripheral blood mononuclear cells (PBMC) from children (age 10-13 years), adults (18-45 years) and the elderly (50-80 years). UCBC or PBMC were stimulated with extracts from A. fumigatus (final concentration 20 μg/ml) (Part A) or D. farinae (20 μg/ml) and D. pteronyssinus (20 ug/ml) (Part B) for 48 hours at 37%, 5% CO2 and saturated humidity. Cytokine and chemokine concentrations in cell culture supernatant were determined by enzyme linked immunosorbent assay (ELISA). The means of the brut production and the lower and upper 95% confidence intervals (in brackets) are shown. ND Not determined.