Cellular production of interleukin (IL)-19, IL-27, IL-33 MCP-4/CCL13, MIP-1delta/CCL15 and Eotaxin-2/CCL24 in response to allergen extracts by umbilical cord blood cells from neonates (UCBC) or peripheral blood mononuclear cells (PBMC) from children (age 10-13 years), adults (18-45 years) and the elderly (50-80 years). UCBC or PBMC were stimulated with extracts from A. fumigatus (final concentration 20 μg/ml) (Part A) or D. farinae (20 μg/ml) and D. pteronyssinus (20 ug/ml) (Part B) for 48 hours at 37%, 5% CO2 and saturated humidity. Cytokine and chemokine concentrations in cell culture supernatant were determined by enzyme linked immunosorbent assay (ELISA). The means of the brut production and the lower and upper 95% confidence intervals (in brackets) are shown. ND Not determined.