Figure 2From: Increased activation and cytokine secretion in B cells stimulated with leptin in aged humansLeptin-induced secretion of cytokines. Purified B cells from young and aged were cultured in the absence or presence of leptin (25 ng/ml and 50 ng/ml) for 24 hours and supernatants were analyzed for TNF-α, IL-6, and IL-10 using ELISA assay. Data are expressed as mean ± sd. Each experiment was done in triplicates.Back to article page