Figure 4
From: Increased activation and cytokine secretion in B cells stimulated with leptin in aged humans
Leptin-induced phosphorylation of STAT3 in B cells. Purified B cells from young and aged were cutlured in the presence and absence of 50 ng/ml leptin for 10 min and native and phosphorylated STAT3 were analyzed by Western blot using specific antibodies. Actin was used an an internal loading control. Quantitation of bands was performed by densitometry and shown in table below.