Figure 1
Decreased response of T cells from aged Clone-4 mice after stimulation with HA 518-524 . 1x105 CFSE labeled splenocytes of young and aged Clone-4 mice (Thy-1.1+) were stimulated with or without 0.1 μM HA518-524 (+ or -). On Days 1, 2, and 3, cells were stained with anti-CD8 antibody. (A). Each flow cytometric plot shows proliferation of CD8 T cells based on the loss of CFSE. (B). Overlay of CFSE profiles of CD8 T cells from young and aged Clone-4 mice. Results are representative of three independent experiments with similar results.