Figure 2

Impact of altered CD90 expression with aging on the specific T cell response. (A). Splenocytes of naïve young and aged Clone-4 mice were stained with HA_518-524 tetramer, anti-CD8, and Thy-1.1 antibodies and gated on CD8⁺ cells. (B). 1x10⁵ CFSE labeled-splenocytes of aged Clone-4 mice were stimulated with or without HA_518-524. On Days 1, 2, and 3, cells were stained with anti-CD8, Thy-1.1, CD69, and CD25 antibodies. The proliferation and early activation of CD90^hi and CD90^low cells are presented in each plot. Green line: Tg T cells/-HA; red line: Thy1.1^hi/+HA; and blue line: Thy-1.1^low/+HA. The experiment was performed three times with similar results.