Figure 4
Enhanced CD90 expression on CD8 T cells after stimulation in vitro . Splenocytes of young and aged Clone-4 mice were cultured with or without HA518-524. (A). On Days 1, 2, and 3, cells were stained with anti-CD8 and Thy-1.1 antibodies. The expression of CD90 on the CD8 T cells are presented in each plot. Blue line: +HA; red line: -HA. (B). MFI of CD44 on CD90hi (red) and CD90low (blue) on Day 1 after stimulation. The number represents MFI of CD90 on the transgenic CD8 T cells. The experiment was performed three times with similar results.