Figure 2

BrdU-flowFISH allows for TL measurement in proliferating CD4⁺and CD8⁺T lymphocytes. (A) Flow cytometry gating strategy for TL measurement from probe mean fluorescence intensity (MFI) in BrdU⁺ CD4⁺ and CD8⁺ cells and in BrdU-negative naïve (CD45RA⁺) T cells. (B) Representative proliferative responses (BrdU+ FSC-A^high population) of CD4+ and CD8+ T cells to viral antigen. Values shown are frequencies of CD4⁺ or CD8⁺ T cells that were BrdU+. (C) Histograms of TL distribution for virus-specific CD4⁺ T cells defined as in B. Mean and median fluorescence intensity values, in arbitrary units (AU), are shown for each plot. Coefficient of variation (CV) is also shown.