Figure 4

TL in CMV- and VACV-specific CD4⁺T cells are longer than TL in IAV-specific CD4⁺T cells. (A) Frequencies of BrdU+CD4⁺ T cells in ten healthy adults. (B) Mean TL measured in CD4⁺ T cells grouped by subject in units of molecules of equivalent soluble fluorescence (MESF). (C) Absolute TL in CD4⁺ T cells grouped by virus. (D) TL in CD4⁺ T cells that proliferated to viral antigen normalized to TL in naïve CD4⁺ T cells (BrdU^negative CD45RA⁺) (TL/TL_naïve) in the same subject. Statistical analyses: ** p < 0.01, * p < 0.05 by Wilcoxon paired, signed rank test. (E) Linear regression analyses for correlations between virus-specific proliferation frequencies (% BrdU+) and TL/TL_naive. P values are from Pearson correlation and linear regression testing. For CMV, n=9; all others, n=10.