Figure 5

VACV-specific memory CD4⁺T cells have a higher frequency of CD45RA⁺cells with long telomeres. (A) CD4⁺ T cell proliferative responses (BrdU⁺) gated by CD45RA^+/−from 1 subject. Histograms of TL in CD45RA^- and CD45RA⁺ cells are overlaid, and mean and median fluorescence intensities for each population are shown. (B) Mean and median telomere probe fluorescence intensity values for all subjects’ grouped by virus. Box and whisker plots indicate minimum to maximum values with the lines inside the boxes depicting the median values for each set of measurements. (C) Frequencies of BrdU⁺ CD45RA⁺ CD4⁺ T cells with long telomeres in one subject. (D) Frequency of CD45RA⁺ CD4⁺ T cells with long telomere within the BrdU+ population for 10 subjects. * p < 0.05 by Wilcoxon paired, signed rank test. Fluorescence is shown in arbitrary units (AU).