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Figure 3 | Immunity & Ageing

Figure 3

From: Acute exercise decreases PTP-1B protein level and improves insulin signaling in the liver of old rats

Figure 3

Effect of acute exercise on PTP-1B protein levels and PTP1B association with IRβ and IRS-1. A, PTP-1B protein level in Old and Old Exe rats were compared with Young group. B, tissue extracts were immunoprecipitated (IP) with anti-IRβ followed by immunoblotting (IB) with anti-PTP1B antibody or anti-IRβ antibody (upper and lower panels). C, IP with anti-IRS-1 followed by IB with anti-PTP1B antibody to evaluated the IRS-1–PTP1B association (upper panel). Liver extracts were also IP with anti-IRS-1 and IB with anti-IRS-1 antibody (lower panels). Immunoblot was performed employing anti-β-Actin antibody as the loaded protein (lower panels in A). The results of scanning densitometry were expressed as arbitrary units. Bars represent means ± S.E.M. of n = 6 rats. ∗P < 0.05, versus Young and #P < 0.05, versus Old.

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