CD11c+dendritic cell proportions increase in elderly murine tissues. Murine tissues were harvested, disaggregated into single cell suspensions and stained for CD11c+ DCs. CD11c+ DCs were identified and gated relative to the isotype control using flow cytometry (A). The percentages of cells positive for CD11c in tissues are shown in young and elderly mice (B). Data is shown as individual values (dots) and mean ± SEM, and is pooled from 2 separate experiments, total n = 7 – 8 mice/group. * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001; **** = p ≤ 0.0001.