Neutral lipid content is influenced by age and DC subpopulation in mesLNs and livers. The lipid content of DC subpopulations from murine mesLNs (A) and livers (B) was evaluated by flow cytometry following BODIPY staining. Representative data from 1 of 2 experiments is shown as individual MFIs and mean ± SEM, total n = 7 – 8 mice/group. Significance was calculated using two-way ANOVA. * = p ≤ 0.05; ** = p ≤ 0.01; *** = p ≤ 0.001; **** = p ≤ 0.0001.