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Figure 6 | Immunity & Ageing

Figure 6

From: The effect of aging on the frequency, phenotype and cytokine production of human blood CD4 + CXCR5 + T follicular helper cells: comparison of aged and young subjects

Figure 6

Comparison of IFN-γ+, IL-17+, IL-4 + or IL-22+ cells among aged and young CXCR5 + CD4 + Tfh. PBMCs were prepared, stimulated with PMA and inomycin for 4–6 hours, and intracellular staining were done. FACS data were analyzed: lymphocytes were first gated and then CD4 + CXCR5+ or CD4 + CXCR5- cells were gated and cytokines such as IFN-γ, IL-17, IL-4 and IL-22 were analyzed. Statistical data of frequencies of IFN-γ + (A), IL-17 + (B), IL-4 + (C) and IL-22 + (D) among CD4 + CXCR5 + Tfh or CD4 + CXCR5- cells were shown. *: p < 0.05, n.s: no significant difference. All data were expressed with line at mean with SEM.

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