Different frequencies of IFN-γ+, IL-17+, IL-4+ or IL-22+ cells among Tfh compared with CD4 + CXCR5- cells. Statistical data of frequencies of IFN-γ + (A), IL-17 + (B), IL-4 + (C) and IL-22 + (D) among CD4 + CXCR5 + Tfh or CD4 + CXCR5- cells were shown. All data were expressed with line at mean with SEM. Differences between two groups were assessed by paired t test for two-tails. *: p < 0.05, n.s: no significant difference.