Figure 5
No local proliferation, nor preferential Treg induction in ageing GALT. (A) Proliferative status of CD4 T cells in the GALT. Percentage of Ki67 expressing cells were determined on CD4 T cells recovered from PP, siLP and cLP from young (Y) (n = 24), middle-aged (M) (n = 12) and old (O) (n = 10) C57BL/6 mice. (B) Proportion of FOXP3 non-expressing (Tconv) and FOXP3 expressing (Treg) CD4 T cells in GALT from young (n = 25), middle-aged (n = 15) and old (n = 10) C57BL/6 mice. Cumulative results show the mean ± SEM of absolute numbers recovered from independent experiments comparing young, middle-aged and aged animals. Statistical significance (Student’s t test) is shown: ns, non-significant; *, p < 0.05; **, p < 0.01.