Figure 3
From: Impact of ageing on the response and repertoire of influenza virus-specific CD4 T cells
The T cell receptor Vβ repertoire of influenza NP-specific young and aged CD4 T cells. Ten days after i.n. infection with 3,000 EID50 ×-31 influenza virus, the repertoire of NP311–325/IAb-specific CD4 T cells in the lungs of mice was assessed by T cell receptor Vβ staining of tetramer-positive CD4 T cells. Panel A shows repertoire analysis of NP311–325/IAb-specific CD4 T cells from the lungs of individual influenza virus-infected young mice (n = 5) using the full panel of anti-Vβ antibodies. Pan 8 signifies Vβ8.1, 8.2 and 8.3. Values for each Vβ are represented by a different symbol. Panel B shows selected T cell receptor Vβ repertoire analysis of NP311–325/IAb-specific CD4 T cells from the lungs of individual young (closed circles, n = 5) and aged (open circles, n = 10) mice 12 days post infection. Variances were compared by F test (***p < 0.0005). Group means are indicated by bars.