Figure 5

Age-associated changes in T cell receptor Vβ distribution in CD4 and CD8 naïve and activated/memory T cells in specific pathogen free mice. T cell receptor Vβ usage was assessed among distinct populations of CD44^high (activated/memory) or CD44^low (naïve) CD4 and CD8 T cells in individual young and aged mice. The proportion of cells from 5 young or 10 aged mice bearing individual T cell receptor Vβ elements was determined by staining with a panel of anti-Vβ antibodies, and is represented by different colors, which are the same order in the key and individual samples. Panel A shows the Vβ distribution among CD44^high CD8 T cells. Panel B shows the Vβ distribution among CD44^high CD4 T cells. Panel C shows the Vβ distribution among CD44^low CD8 T cells. Panel D shows the Vβ distribution among CD44^low CD4 T cells. Statistical analysis is described in the text.