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Figure 5 | Immunity & Ageing

Figure 5

From: Heparan sulfate proteoglycan induces the production of NO and TNF-α by murine microglia

Figure 5

Effect of heat denaturated HSPG and HS-GAG chains on iNOS and TNF-α mRNA expression in murine microglia. Microglial cells were cultured in 48-well plates and then stimulated for 4 hours with 15 μg/ml HSPG, 15 μg/ml heat denaturated HSPG (HSPG-hd), 15 μg/ml HS-GAG chains (HS) or 100 ng/ml LPS. Total RNA was extracted and analysed by RT-PCR. The densitometric evaluation of the bands obtained from three independent experiments in duplicates are reported (n = 6); values are expressed as the relative level of iNOS/GAPDH and TNF-α/GAPDH. *p < 0.05.

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