Table I Oligonucleotide Primers Used for cDNA amplification
From: Heparan sulfate proteoglycan induces the production of NO and TNF-α by murine microglia
Probe | Cycles | Orientation | Tann | Sequence | Length of PCR Fragments (bp) |
|---|---|---|---|---|---|
GAPDH | 20 | sense antisense | 68 | 5'TGAAGGTCGGTGTGAACGGATTTGGC3' 5'CATGTAGGCCATGAGGTCCACCAC3' | 983 |
iNOS | 26 | sense antisense | 68 | 5'CCCTTCCGAAGTTTCTGGCAGCAGC3' 5'GGCTGTCAGAGCCTCGTGGCTTTGG3' | 493 |
TNFα | 24 | sense antisense | 60 | 5'TTCTGTCTACTGAACTTCGGGTGATCGGTCC3' 5'GTATGAGATAGCAAATCGGCTGACGGTGTGGG3' | 378 |