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Figure 1 | Immunity & Ageing

Figure 1

From: Constitutive degradation of IκBα in human T lymphocytes is mediated by calpain

Figure 1

Effect of treatment with E64D on constitutive levels of IκBα in T cells from young and elderly donors. T cells obtained from young and elderly donors were either treated with E64D (+), 50 μM, or left untreated (-) for 45 minutes. At the end of incubation, cells were washed and cell lysates prepared. Lysates, equalized for protein, were resolved using SDS-PAGE, followed by electroblotting. Resolved proteins were detected using specific antibody to IκBα and ECL. Representative data from one donor pair are presented (A). Values obtained from a minimum of 4 donor pairs following densitometric scanning, are presented as mean integrated density units ± S.D.(B); ** indicates statistical difference from untreated controls.

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