Figure 3
From: Constitutive degradation of IκBα in human T lymphocytes is mediated by calpain
Effect of age on constitutive degradation of IκBγ. T cells obtained from young and elderly donors were either treated with E64D (+), 50 μM, or left untreated (-) for 45 minutes. At the end of incubation, cells were washed and cell lysates prepared. Lysates, equalized for protein, were resolved using SDS-PAGE, followed by electroblotting. Resolved proteins were detected using specific antibody to IκBγ and ECL. Representative data from one donor pair are presented (A). Values obtained from a minimum of 4 donor pairs following densitometric scanning, are presented as mean integrated density units ± S.D.(B).