Skip to main content
Figure 8 | Immunity & Ageing

Figure 8

From: Constitutive degradation of IκBα in human T lymphocytes is mediated by calpain

Figure 8

Basal Calpain activity in T cells obtained from young and elderly donors. T cells obtained from young and elderly donors were adjusted to 2.5 × 105 cells /ml. Calpain activity was measured using a fluorogenic substrate Boc-Met-AMC at 380 nm exc and 460 nm emm using a fluorometer. Specific activity was determined in the presence of calpain inhibitor E64D. Values represent calpain specific activity and represent Mean fluorescence intensity ± S.D of data obtained from 4 independent experiments.

Back to article page