The role of the MAPK pathway alterations in GM-CSF modulated human neutrophil apoptosis with aging

Immunity & Ageing

Table 2 Modulation of caspase-3 activity in PMN by MAPkinase inhibitors

Treatment	Culture time (hours)	Caspase-3 activity (arbitrary fluorescence units)
		Young	Elderly
	0	92 ± 20	126 ± 29
None	6	218 ± 38	188 ± 53
	18	908 ± 118	1396 ± 207*
GM-CSF	6	113 ± 31	149 ± 42
	18	447 ± 87	979 ± 96*
PD98059	18	781 ± 216	1024 ± 413
GM-CSF + PD98059	18	1126 ± 376	1145 ± 387
SB203580	18	1055 ± 267	792 ± 291
GM-CSF + SB203580	18	459 ± 119	900 ± 147 *

PMN isolated from young and elderly donors were maintained in culture for the indicated periods of time. The cells were left untreated, or exposed to GM-CSF (20 ng/ml) or a combination of GM-CSF (20 ng/ml) and either in the presence or absence of PD98059 (30 μM) or SB203580 (10 μM). Caspase-3 activity was measured using a fluorescent substrate. Data are representative of 10 independent experiments. The asterisk (*) indicate significant differences (p < 0.05) between the two groups of donors.

ISSN: 1742-4933