Figure 1

IL-1α expression in HUVEC. (A) 3 μg of polyA⁺ RNA were utilized for Northern analysis. GAPDH hybridization shows that comparable amounts of RNA were loaded per lane. P: proliferating cells (PD 20); Q: quiescent cells (PD 20). PD indicates the number of population doublings undergone by HUVEC (from young -PD 13- to senescent – PD 45-). (B) Cell lysates (450 μg) from proliferating vs quiescent HUVEC were separated by a 15% SDS-PAGE and western blot was performed as described in the methods. After stripping, the blot was incubated with an anti-actin antibody to show that comparable amounts of protein were loaded per lane. P: proliferating cells; Q: quiescent cells, both at PD 20.