Figure 2
From: Iterleukin 1 alpha is a marker of endothelial cellular senescent
IL-1α total amounts in young and senescent HUVEC. Cell lysates (350 μg) from young and senescent HUVEC were separated by a 15% SDS-PAGE and western blot was performed as described. After stripping, the blot was incubated with an anti-actin antibody to show that comparable amounts of protein were loaded per lane. PD 15: young HUVEC; PD 45: senescent HUVEC.