Figure 3
From: Iterleukin 1 alpha is a marker of endothelial cellular senescent
IL-1α, IL-1β and IL-1ra expression in young and senescentHUVEC. RNA was extracted from HUVEC at different PD (13, 31,54). RT-PCR was performed using specific primers for IL-1α, IL-1β and IL-1ra. PCR amplification was stopped after 15 and 30 cycles to provide a better quantification of the results. GAPDH was utilized to show that similar amounts of RNA had been reverse-transcribed.