Figure 4
From: Iterleukin 1 alpha is a marker of endothelial cellular senescent
IL-1α expression in young, senescent and progeric human dermal fibroblasts. (A) 3 μg of polyA+ RNA were utilized for Northern analysis. GAPDH hybridization shows that comparable amounts of RNA were loaded. PD 9: young fibroblasts; PD 43: senescent fibroblasts. Two different progeric cell types and age-matched controls have been utilized as described. Progeric and control #1: GM0498 and AG6917, respectively; progeric and control #2: GM2037 and AG3513, respectively. (B) Cell lysates (250 μg) from young, senescent and progeric (#1: GM0498 and AG6917) dermal fibroblasts were separated by a 15% SDS-PAGE and western blot was performed. After stripping, the blot was incubated with an anti-actin antibody to show that comparable amounts of protein were loaded per lane. PD 9: young fibroblasts; PD 43: senescent fibroblasts; progeric and control #1: GM0498 and AG6917.