Figure 5
From: Iterleukin 1 alpha is a marker of endothelial cellular senescent
IL-1β and IL-1ra expression in young, senescent and progeric human dermal fibroblasts. RNA was extracted to perform an RT-PCR using specific primers for IL-1β and IL-1ra. GAPDH was utilized to show that comparable amounts of RNA had been reverse-transcribed. 30 cycles of PCR were performed. PD 9: young fibroblasts; PD 43: senescent fibroblasts; progeric and control #1: GM0498 and AG6917.