Figure 3
From: Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels
A) 1, 2 and 4 μg of 16HBE cell extracts (lanes 1, 2, and 3, respectively) were fractionated on a 10% H-SDS-PA gel and probed with mouse anti HSP27 monoclonal antibody as described in Materials and Methods. B) 0.5, 2.5 and 12.5 μg of Parj2 E. Coli lysate (lanes 4, 5, and 6, respectively) were fractionated on a 10% H-SDS-PA gel and probed with pooled allergic human sera, as described in Materials and Methods.