Figure 3

Stimulation of PARP activity in permeabilised human PBMC by addition of NAD and activator oligonucleotide, and inhibitory effect of 3-aminobenzamide. A. Histograms of poly(ADP-ribosyl)ation levels determined using Protocol B. Permeabilised cells were incubated with NAD⁺ and activator oligonucleotide in the presence of the PARP inhibitor 3-aminobenzamide as indicated. A representative set of data is shown. bckgr, background; ab, antibody; 3-AB, 3-aminobenzamide. Note that a shift to the right (FL-1; x-axis) indicates increased levels of poly(ADP-ribosyl)ation and that addition of PARP inhibitor 3-aminobenzamide reverses this shift. B. Quantitative evaluation of MFI. Shown are average values and SD of three parallel determinations, respectively, comprising the data set shown in A