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Table 1b Robustness of poly(ADP-ribosyl)ation capacity measurements of PBMC from one donor done either in parallel or consecutive assays.

From: Flow-cytometric assessment of cellular poly(ADP-ribosyl)ation capacity in peripheral blood lymphocytes

parallel measurements

MFI

 

220

 

190

 

165

 

269

 

210

 

197

Average

208

SD

35

consecutive measurements

MFI

 

193

 

201

 

323

Average

239

SD

73

  1. Cellular poly(ADP-ribosyl)ation capacity was measured using Protocol B. Top: Six independent reactions were started with PBMC from one donor within a single experiment. Bottom: The poly(ADP-ribosyl)ation capacity of one donor was measured in PBMC from blood samples obtained on different days. Note the relatively small variability of the measurements in contrast to samples from different donors. MFI, mean fluorescence intensity; SD, standard deviation.