Sequence analysis of the CDR3-region of BV8+ CMV
-specific T cells from different age-groups. PCR-products were generated of the relevant TCR-sequences and cloned into a bacterial vector. 4 arbitrarily chosen bacterial clones are shown for each donor. Two dominant sequences and a variety of individually used sequences were detected. Conserved sequences were categorized as "SANYGYT" (light grey) and "SVNEAF" (dark grey). Discordance of one or two amino acids from the conserved dominant sequences was considered as minor variation, and these clones were included in these two categories. Sequences with greater variations were considered as private clones.